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How does Ultraviolet work?
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Strong sunlight disinfects water by permanently de-activating
bacteria, spores, moulds and viruses. Over a century ago, scientists identified
the part of the electromagnetic spectrum responsible for this well-known effect;
wavelengths between 200nm and 300nm, often called UV-C. The most effective single
wavelength is typically UV at 265nm, however recent research in the USA has shown
that 271nm light and 263nm light are the most effective UV wavelengths for the
deactivation of particular target organisms.
The mechanism of elimination is well documented and unlike chemical
disinfectants the organism is unable to develop any immune mechanisms. The
mechanism of elimination involves the absorption of photons of UV energy by
the DNA, which fuses the DNA and prevents replication. DNA (Deoxyribonucleic acid)
consists of a linear chain of nitrogen bases known as purines (adenine and guanine)
and pyrimidines (thymine and cytosine). These compounds are linked along the chain
by sugar-phosphate components. The DNA of most forms of life is double stranded
and complimentary; the adenine in one strand is always opposite thymine in the
other, and linked by a hydrogen bond, and guanine is always paired with cytosine
by a hydrogen bond. The purine and pyrimidine combinations are called base pairs.
When UV light of a germicidal wavelength is absorbed by the pyrimidine bases
(usually thymine) the hydrogen bond is ruptured. The dimer that is formed links
the two bases together, and this disruption in the DNA chain means that when the
cell undergoes mitosis (cell division) the DNA is not able to replicate. The most
effective wavelengths to achieve this effect are found between 263nm to 275nm, and
the peak wavelength distribution is dependent on the target organism.
Research is now being undertaken to verify the effect that shorter
wavelength UV light has on the other cell membrane of the organism. This shorter
wavelength is more energetic and is absorbed by the organisms outer membrane.
A lethal insult is effected, which means that the cell is unable to effectively
regulate osmotic pressure. This effect coupled with the fusing of the DNA means
that UV is a simple, elegant disinfectant and one that will increasingly replace
the more traditional chemical techniques.
Water enters the purifier's chamber. Once inside, it is exposed to UV
light. The UV lamp used for this type of germicidal disinfection produces light
at a wavelength of 253.7 nanometers (2,537 Angstrom units). At this wavelength,
UV light can destroy up to 99.99% of all bacteria, protozoa, viruses, molds, algae
and other microbes.
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